Journal: Mediterranean Journal of Rheumatology
Article Title: Evaluation of IRF7 mRNA and its Association with Promoter Methylation in Kashmiri (North-Indian) Patients with Systemic Sclerosis: A Case-Control Study
doi: 10.31138/mjr.040124.eoi
Figure Lengend Snippet: (A) Methylation-specific PCR results of IRF-7 promoter using specific primers. Lane MM: 50bp in DNA molecular weight marker; Lane M: Methylated band (196bp); Lane U: Unmethylated band (208bp); Lane Mc: Human Methylation (+) DNA Control; Lane Uc: Human Unmethylation (−) DNA Control. (B) Association of methylation status with cases and controls in systemic sclerosis. (C) Effect of IRF7 promoter methylation on its mRNA Expression. **P=<0.01; ***P=<0.001.
Article Snippet: To verify the PCR results, representative bands from each target and housekeeping gene Human methylated DNA control and Human non-methylated control (ZYMO Research) were gel-purified on a 2% agarose gel yielding a band of 196bp for methylated and 208bp for an unmethylated product and visualised under UV illumination.
Techniques: Methylation, Molecular Weight, Marker, Control, Expressing